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Flow cytometric cell sorting Cells were resuspended in cold staining buffer (PBS with 2% FBS) at a density of 1 × 10 7 cells/mL and incubated with a fluorochrome-conjugated anti-GLUT1 antibody for 30 min at 4 °C in the dark, using an isotype-matched antibody to define negative gating

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Operational and Label Literacy: COA Reading and Reconstitution Math Reading a Certificate of Analysis for DSIP HPLC purity: Look for greater than 98% by HPLC