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References Rat anabolic/nitrogen retention dosing — including des(1‑3) IGF‑1 ~1.08 mg/kg/day Nephrectomized rat model — des(1‑3) IGF‑1 0.9 mg/kg/day for 7 days Des(1‑3) IGF‑1 additional rat dosing example — 1.2 mg/kg/day In‑vitro concentration range example — 6.7–53 nM Biodistribution/clearance considerations — for labeled des(1‑3) IGF‑1 Research Resources Follistatin 344 (FS344) — Human dosage only (what's actually in clinical studies) Quickstart Highlights Compound (Human clinical form): rAAV1.CMV.huFS344 (AAV1‑mediated follistatin FS344 gene transfer) Route used in trials: Intramuscular (IM) injections into targeted muscles How dosing is expressed: vector genomes per kilogram (vg/kg) (not mg) Human trial doses reported: 6 × 10¹¹ vg/kg and 1.2 × 10¹² vg/kg (BMD cohorts)

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