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To assess whether vitamin B 12 was covalently bound to a particular peptide, other samples of red mAb X and mAb Y BDS were prepared as described previously 16 and buffer exchanged into 25 mM Tris, 2 mM CaCl2, pH 8.0 for tryptic digests or 50 mM ammonium bicarbonate, pH 8.0 for endoproteinase Glu-C digests
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