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The western blot was performed using a GPX4 antibody (Abcam, [EPNCIR144], ab125066) (1:1000), β-actin antibody (Abcam, ab8227) (1:5000) for loading control, and goat anti-rabbit IgG (H + L) secondary antibody, HRP (ThermoFisher Scientific, 31460) (1:3000)

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This is why so many feel fatigued, gain weight so easily, or lose weight no longer even when dieting