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The earliest subjective improvements after B12 treatment occurred for paresthesia and balance, leading to full recovery [87]

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The resulting cDNA was used as a template for quantitative RT-PCR, which was performed with primers specific for TGF-β1 and β-actin (see in Table 1 ), according to the instructions for the SYBR Premix EX Taq Kit (TaKaRa Biotechnology Co., Ltd.), and a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, United States)