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For instance, NAD + levels and mitochondrial function have been shown to decline in sarcopenia muscle in one cohort 249 , while another study did not observe a difference in NAD + levels in sarcopenic muscle, and even NR supplementation had no effect on NAD + levels 262
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Alternative approaches to obtain a preparation with an intact brush border include the use of different sized sieves to separate glomeruli and tubules (Bach et al., 1986), which may not result in a pure preparation of proximal tubular cells
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