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Briefly, AT2 cells were seeded in 96-well plates (5,000 cells/well or 6-well plates (1×105 cells/well), pretreated with quercetin (0, 5, 10 and 20 μM) for 2 h, and then LPS (5, 10 or 20 μg/ml) was used to induce the acute cell injury model

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These factors are especially true for the association between hyperhomocysteinemia and stroke