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According to the digestion protocol, 50 μg of cell lysate was mixed with 10% SDS, 500 mM DTT, 1 M Tris HCl, pH 8.0 and incubated at 650 rpm for 10 min at 95°C
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There are two catalytic pathways for dual-substrate reactions: ping-pong reactions, where one product is released before all substrates bind (Fig
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