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N-terminally Strep-MBP-tagged and C-terminally FLAG-tagged EBOV L (hereafter referred to as EBOL), and N-terminally His 6 -MBP-tagged eVP35 for EBOV L–VP35 complex (Supplementary Fig

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Shaking the vial (denatures peptides), injecting water too quickly (creates foam), using the wrong volume because the math was done by hand instead of a peptide dosage calculator, storing reconstituted peptides at room temperature, freeze-thaw cycles, and failing to label vials with concentration and date