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Following centrifugation (20,000 x g, 5 min top remove undigested material), 250 µl of each sample was transferred into a new tube containing 250 µl of 1 st wash buffer (50 mM TEAB, 150 mM NaCl, 0.2% NP-40) containing 50 µl of streptavidin agarose beads that were pre-washed twice by the 1 st wash buffer)

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