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Liver tissues (50 mg) and cIEC pellets harvested from colon tissues (~7 cm) were homogenized with sterile grinding beads in 500 μL PBS at 45 Hz for 1 min, centrifuged (6000 × g , 5 min, 4 °C) to collected the supernatant and divided in two aliquots for total protein and BHB measurement

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