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Anulus Fibrosus The fibrocartilaginous AF is composed largely of highly oriented collagen fibers (principally type I collagen) organized into alternately oriented lamellae (50), that resist the high tensile stresses generated during pressurization of the central NP and during flexion-extension and lateral bending

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This leads to the release and subsequent cytoplasmic translocation of p53, which in turn activates a transcription-independent pro-apoptotic pathway, facilitating the selective clearance of senescent cells (Kong et al., 2025