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Mechanistic studies have shown that EESP induces apoptosis and inhibits cell proliferation and angiogenesis through inhibition of STAT3 phosphorylation, upregulation of the pro-apoptotic protein Bax/Bcl-2 ratio, downregulation of the cell cycle proteins Cyclin D1 and CDK4, and reduction of VEGF-A and VEGFR-2 ( Convallatoxin a natural triterpenoid derived from Adonis amurensis Regel & Radde , has significant anticancer activity ( In vitro experiments showed that Convastatin (0–100 nM) significantly downregulated STAT3 phosphorylation levels by inhibiting the interaction of JAK2/STAT3 and mTOR/STAT3 signaling pathways, thereby inhibiting the proliferation, migration and invasion of CRC cells and inducing apoptosis
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