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Samples (in 40 mM PBS, pH 7.0) were analyzed by capillary electrophoresis (voltage = 30 kV, detection at 195 nm, Agilent 7100, Agilent, Santa Clara, CA, USA) equipped with a polyimidecoated fused silica capillary (68 cm x 50 μm) as described previously.22 High-resolution respirometry The respiration of intact and treated cells in the complete medium was measured with the Oroboros Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria)
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A genome-wide DNA methylation profiling study showed that Fgf16 , Fgf17 , Fgf20 and Fgfr4 are highly methylated in cultured astrocytes (Meissner et al., 2008)
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